منابع مشابه
Studies on Cytochrome Oxidase
Ever since cytochrome oxidase was first solubilized from heart muscle preparations by Yakushiji and Okunuki (1) and Straub (2) in 1941, the further purification of this enzyme has been the subject of studies by many workers, using extraction with bile salts (3-14), or digestive enzymes such as trypsin (6, 7) and snake venom (15), fractionation with ammonium sulfate (5-14), chromatography (9, 12...
متن کاملStudies on cytochrome oxidase. IV. The cytochrome oxidase activity.
where the activity is determined by measuring the rate of oxidation of reduced cytochrome c. Smith (6, 7), Conrad (8, 9), Wainio (10-13) et ~2. have extensively investigated the effect of various factors on the cytochrome oxidase activity measured by System 2 with soluble and insoluble preparations of cytochrome oxidase, whereas among those (14-30) who have employed System 1, Slater (5), using ...
متن کاملStudies on Cytochrome Oxidase. Vi. Kinetics of the Aerobic Oxidation of Ferrocytochrome C by Cytochrome Oxidase.
The rate of reaction can be determined by measuring the rate of either oxygen consumption or ferrocytochrome c oxidation. When the rate of oxygen consumption is measured either manometrically or polarographically, a mixture of a limited amount of cytochrome c and an excess of reducing agent, instead of excess ferrocytochrome c, is used as an electron donor system. In this case the true electron...
متن کاملStudies on cytochrome oxidase in carbon tetrachloride treated rats.
Cytochrome c oxidase was purified from control and CCl4 treated rats and its kinetic properties were studied. The activity of the enzyme was inhibited by 51% in CCl4 (4 g per kg body weight for 24 hr) treated rats. Studies on the kinetic properties showed that the K(m) of the enzyme increased by 60% while Vmax decreased by 44% in CCl4 treated rats compared to controls. The content of cytochrome...
متن کاملElectron paramagnetic resonance studies on the cytochrome oxidase from yeast.
I M. D. KAMEN, R. G. BARTSCH, T. HORIO AND H. DE KLERK, in S. P. COLO\VICK AND N. O. tXAPLAN, Methods in Enzymology, Vol. VI, Academic Press, New York, I963, p. 391. 2 T. HORIO AND M. D. KAMEN, Biochim. Biophys. Acta, 48 (I96I) 266. 3 G. COHEN-BAZIRE, ~V. R. SISTROM AND R. Y. STANIER, J . Cellular Physiol., 49 (1957) 25. 4 R. G. BARTSCH, in H. (;EST AND A. SAN PIETRO, Bacterial Photosynthesis, ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84009-7